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Expression vector

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An expression vector, otherwise known as an expression construct, is usually a <a href="Plasmid" title="Plasmid">plasmid</a> or virus designed for <a href="Gene_expression" title="Gene expression">gene expression</a> in cells. The <a href="Vector_(molecular_biology)" title="Vector (molecular biology)">vector</a> is used to introduce a specific <a href="Gene" title="Gene">gene</a> into a target cell, and can commandeer the cell's mechanism for <a href="Protein_synthesis" class="mw-redirect" title="Protein synthesis">protein synthesis</a> to produce the <a href="Protein" title="Protein">protein</a> <a href="Genetic_code" title="Genetic code">encoded</a> by the gene. Expression vectors are the basic tools in <a href="Biotechnology" title="Biotechnology">biotechnology</a> for the <a href="Protein_production" title="Protein production">production of proteins</a>.

The <a href="Vector_(molecular_biology)" title="Vector (molecular biology)">vector</a> is engineered to contain regulatory sequences that act as <a href="Enhancer_(genetics)" title="Enhancer (genetics)">enhancer</a> and <a href="Promoter_(biology)" class="mw-redirect" title="Promoter (biology)">promoter</a> regions and lead to efficient transcription of the gene carried on the expression vector.<a href="#cite_note-1">[1]</a> The goal of a well-designed expression vector is the efficient production of protein, and this may be achieved by the production of significant amount of stable <a href="Messenger_RNA" title="Messenger RNA">messenger RNA</a>, which can then be <a href="Translation_(biology)" title="Translation (biology)">translated</a> into protein. The expression of a protein may be tightly controlled, and the protein is only produced in significant quantity when necessary through the use of an <a href="Inducer" title="Inducer">inducer</a>. In some systems, however, the protein may be expressed constitutively. <a href="Escherichia_coli" title="Escherichia coli">Escherichia coli</a> is commonly used as the host for <a href="Protein_production" title="Protein production">protein production</a>, but other cell types may also be used. An example of the use of expression vector is the production of <a href="Insulin" title="Insulin">insulin</a>, which is used for medical treatments of <a href="Diabetes" title="Diabetes">diabetes</a>.

<div class="mw-heading mw-heading2"><h2 id="Elements">Elements</h2></div>

An expression vector has features that any <a href="Vector_(molecular_biology)" title="Vector (molecular biology)">vector</a> may have, such as an <a href="Origin_of_replication" title="Origin of replication">origin of replication</a>, a <a href="Selectable_marker" title="Selectable marker">selectable marker</a>, and a suitable site for the insertion of a gene like the <a href="Multiple_cloning_site" title="Multiple cloning site">multiple cloning site</a>. The cloned gene may be transferred from a specialized <a href="Cloning_vectors" class="mw-redirect" title="Cloning vectors">cloning vector</a> to an expression vector, although it is possible to clone directly into an expression vector. The cloning process is normally performed in <a href="Escherichia_coli_(molecular_biology)" class="mw-redirect" title="Escherichia coli (molecular biology)">Escherichia coli</a>. Vectors used for protein production in organisms other than E.coli may have, in addition to a suitable origin of replication for its propagation in E. coli, elements that allow them to be maintained in another organism, and these vectors are called <a href="Shuttle_vector" title="Shuttle vector">shuttle vectors</a>.

<div class="mw-heading mw-heading3"><h3 id="Elements_for_expression">Elements for expression</h3></div>

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</style><div role="note" class="hatnote navigation-not-searchable">Further information: <a href="Transcription_(genetics)" class="mw-redirect" title="Transcription (genetics)">Transcription (genetics)</a> and <a href="Translation_(biology)" title="Translation (biology)">Translation (biology)</a></div>

An expression vector must have elements necessary for gene expression. These may include a <a href="Promoter_(genetics)" title="Promoter (genetics)">promoter</a>, the correct translation initiation sequence such as a <a href="Ribosomal_binding_site" class="mw-redirect" title="Ribosomal binding site">ribosomal binding site</a> and <a href="Start_codon" title="Start codon">start codon</a>, a <a href="Termination_codon" class="mw-redirect" title="Termination codon">termination codon</a>, and a <a href="Terminator_(genetics)" title="Terminator (genetics)">transcription termination sequence</a>.<a href="#cite_note-2">[2]</a> There are differences in the machinery for protein synthesis between prokaryotes and eukaryotes, therefore the expression vectors must have the elements for expression that are appropriate for the chosen host. For example, prokaryotes expression vectors would have a <a href="Shine-Dalgarno_sequence" class="mw-redirect" title="Shine-Dalgarno sequence">Shine-Dalgarno sequence</a> at its translation initiation site for the binding of ribosomes, while eukaryotes expression vectors would contain the <a href="Kozak_consensus_sequence" title="Kozak consensus sequence">Kozak consensus sequence</a>.

The <a href="Promoter_(genetics)" title="Promoter (genetics)">promoter</a> initiates the <a href="Transcription_(genetics)" class="mw-redirect" title="Transcription (genetics)">transcription</a> and is therefore the point of control for the expression of the cloned gene. The promoters used in expression vector are normally <a href="Enzyme_induction_and_inhibition" title="Enzyme induction and inhibition">inducible</a>, meaning that protein synthesis is only initiated when required by the introduction of an <a href="Inducer" title="Inducer">inducer</a> such as <a href="IPTG" class="mw-redirect" title="IPTG">IPTG</a>. Gene expression however may also be constitutive (i.e. protein is constantly expressed) in some expression vectors. Low level of constitutive protein synthesis may occur even in expression vectors with tightly controlled promoters.

<div class="mw-heading mw-heading3"><h3 id="Protein_tags">Protein tags</h3></div>

<div role="note" class="hatnote navigation-not-searchable">Main article: <a href="Protein_tag" title="Protein tag">Protein tag</a></div>

After the expression of the gene product, it may be necessary to purify the expressed protein; however, separating the protein of interest from the great majority of proteins of the host cell can be a protracted process. To make this purification process easier, a <a href="Protein_tag" title="Protein tag">purification tag</a> may be added to the cloned gene. This tag could be <a href="Polyhistidine-tag" class="mw-redirect" title="Polyhistidine-tag">histidine (His) tag</a>, other marker peptides, or a <a href="Fusion_protein" title="Fusion protein">fusion partners</a> such as <a href="Glutathione_S-transferase" title="Glutathione S-transferase">glutathione S-transferase</a> or <a href="Maltose-binding_protein" title="Maltose-binding protein">maltose-binding protein</a>.<a href="#cite_note-3">[3]</a> Some of these fusion partners may also help to increase the solubility of some expressed proteins. Other fusion proteins such as <a href="Green_fluorescent_protein" title="Green fluorescent protein">green fluorescent protein</a> may act as a <a href="Reporter_gene" title="Reporter gene">reporter gene</a> for the identification of successful cloned genes, or they may be used to study protein expression in <a href="Live_cell_imaging" class="mw-redirect" title="Live cell imaging">cellular imaging</a>.<a href="#cite_note-4">[4]</a><a href="#cite_note-5">[5]</a>

<div class="mw-heading mw-heading3"><h3 id="Other_Elements">Other Elements</h3></div>

The expression vector is <a href="Transformation_(genetics)" class="mw-redirect" title="Transformation (genetics)">transformed</a> or <a href="Transfection" title="Transfection">transfected</a> into the host cell for protein synthesis. Some expression vectors may have elements for transformation or the insertion of DNA into the host chromosome, for example the <a href="Agrobacterium" title="Agrobacterium">vir genes</a> for <a href="Plant_transformation_vector" title="Plant transformation vector">plant transformation</a>, and <a href="Integrase" title="Integrase">integrase</a> sites for chromosomal integration .

Some vectors may include targeting sequence that may target the expressed protein to a specific location such as the <a href="Periplasmic_space" class="mw-redirect" title="Periplasmic space">periplasmic space</a> of bacteria.

<div class="mw-heading mw-heading2"><h2 id="Expression/Production_systems">Expression/Production systems</h2></div>

Different organisms may be used to express a gene's target protein, and the expression vector used will therefore have elements specific for use in the particular organism. The most commonly used organism for <a href="Protein_production" title="Protein production">protein production</a> is the bacterium <a href="Escherichia_coli" title="Escherichia coli">Escherichia coli</a>. However, not all proteins can be successfully expressed in E. coli, or be expressed with the correct form of post-translational modifications such as glycosylations, and other systems may therefore be used.

<div class="mw-heading mw-heading3"><h3 id="Bacterial">Bacterial</h3></div>

The expression host of choice for the expression of many proteins is Escherichia coli as the production of heterologous protein in E. coli is relatively simple and convenient, as well as being rapid and cheap. A large number of E. coli expression plasmids are also available for a wide variety of needs. Other bacteria used for protein production include <a href="Bacillus_subtilis" title="Bacillus subtilis">Bacillus subtilis</a>.

Most heterologous proteins are expressed in the cytoplasm of E. coli. However, not all proteins formed may be soluble in the cytoplasm, and incorrectly folded proteins formed in cytoplasm can form insoluble aggregates called <a href="Inclusion_bodies" title="Inclusion bodies">inclusion bodies</a>. Such insoluble proteins will require refolding, which can be an involved process and may not necessarily produce high yield.<a href="#cite_note-6">[6]</a> Proteins which have <a href="Disulphide_bonds" class="mw-redirect" title="Disulphide bonds">disulphide bonds</a> are often not able to fold correctly due to the reducing environment in the cytoplasm which prevents such bond formation, and a possible solution is to target the protein to the <a href="Periplasmic_space" class="mw-redirect" title="Periplasmic space">periplasmic space</a> by the use of an N-terminal <a href="Signal_peptide" title="Signal peptide">signal sequence</a>. Another possibility is to manipulate the redox environment of the cytoplasm.<a href="#cite_note-7">[7]</a> Other more sophisticated systems are also being developed; such systems may allow for the expression of proteins previously thought impossible in E. coli, such as <a href="Glycosylated" class="mw-redirect" title="Glycosylated">glycosylated</a> proteins.<a href="#cite_note-8">[8]</a><a href="#cite_note-9">[9]</a><a href="#cite_note-10">[10]</a>

The promoters used for these vector are usually based on the promoter of the <a href="Lac_operon" title="Lac operon">lac operon</a> or the <a href="T7_phage" title="T7 phage">T7</a> promoter,<a href="#cite_note-11">[11]</a> and they are normally regulated by the lac <a href="Operator_(biology)" class="mw-redirect" title="Operator (biology)">operator</a>. These promoters may also be hybrids of different promoters, for example, the <a href="Tac-Promoter" title="Tac-Promoter">Tac-Promoter</a> is a hybrid of <a href="Trp_operon" title="Trp operon">trp</a> and lac promoters.<a href="#cite_note-12">[12]</a> Note that most commonly used lac or lac-derived promoters are based on the <a href="LacUV5" title="LacUV5">lacUV5</a> mutant which is insensitive to <a href="Catabolite_repression" title="Catabolite repression">catabolite repression</a>. This mutant allows for expression of protein under the control of the lac promoter when the <a href="Growth_medium" title="Growth medium">growth medium</a> contains glucose since glucose would inhibit gene expression if wild-type lac promoter is used.<a href="#cite_note-13">[13]</a> Presence of glucose nevertheless may still be used to reduce background expression through residual inhibition in some systems.<a href="#cite_note-14">[14]</a>

Examples of E. coli expression vectors are the pGEX series of vectors where <a href="Glutathione_S-transferase" title="Glutathione S-transferase">glutathione S-transferase</a> is used as a fusion partner and gene expression is under the control of the tac promoter,<a href="#cite_note-15">[15]</a><a href="#cite_note-16">[16]</a><a href="#cite_note-17">[17]</a> and the pET series of vectors which uses a <a href="T7_phage" title="T7 phage">T7</a> promoter.<a href="#cite_note-18">[18]</a>

It is possible to simultaneously express two or more different proteins in E. coli using different plasmids. However, when 2 or more plasmids are used, each plasmid needs to use a different antibiotic selection as well as a different origin of replication, otherwise one of the plasmids may not be stably maintained. Many commonly used plasmids are based on the <a href="ColE1" title="ColE1">ColE1</a> replicon and are therefore incompatible with each other; in order for a ColE1-based plasmid to coexist with another in the same cell, the other would need to be of a different replicon, e.g. a p15A replicon-based plasmid such as the pACYC series of plasmids.<a href="#cite_note-19">[19]</a> Another approach would be to use a single two-cistron vector or design the coding sequences in tandem as a bi- or poly-cistronic construct.<a href="#cite_note-20">[20]</a><a href="#cite_note-21">[21]</a>

<div class="mw-heading mw-heading3"><h3 id="Yeast">Yeast</h3></div>

A yeast commonly used for protein production is <a href="Pichia_pastoris" class="mw-redirect" title="Pichia pastoris">Pichia pastoris</a>.<a href="#cite_note-22">[22]</a> Examples of yeast expression vector in Pichia are the pPIC series of vectors, and these vectors use the <a href="AOX1" class="mw-redirect" title="AOX1">AOX1</a> promoter which is inducible with <a href="Methanol" title="Methanol">methanol</a>.<a href="#cite_note-23">[23]</a> The plasmids may contain elements for insertion of foreign DNA into the yeast genome and signal sequence for the secretion of expressed protein. Proteins with disulphide bonds and glycosylation can be efficiently produced in yeast. Another yeast used for protein production is <a href="Kluyveromyces_lactis" title="Kluyveromyces lactis">Kluyveromyces lactis</a> and the gene is expressed, driven by a variant of the strong <a href="Lactase" title="Lactase">lactase</a> LAC4 promoter.<a href="#cite_note-24">[24]</a>

<a href="Saccharomyces_cerevisiae" title="Saccharomyces cerevisiae">Saccharomyces cerevisiae</a> is particularly widely used for gene expression studies in yeast, for example in <a href="Yeast_two-hybrid_system" class="mw-redirect" title="Yeast two-hybrid system">yeast two-hybrid system</a> for the study of protein-protein interaction.<a href="#cite_note-25">[25]</a> The vectors used in yeast two-hybrid system contain fusion partners for two cloned genes that allow the transcription of a reporter gene when there is interaction between the two proteins expressed from the cloned genes.

<div class="mw-heading mw-heading3"><h3 id="Baculovirus">Baculovirus</h3></div>

<a href="Baculovirus" class="mw-redirect" title="Baculovirus">Baculovirus</a>, a rod-shaped virus which infects insect cells, is used as the expression vector in this system.<a href="#cite_note-26">[26]</a> Insect cell lines derived from <a href="Lepidopteran" class="mw-redirect" title="Lepidopteran">Lepidopterans</a> (moths and butterflies), such as <a href="Spodoptera_frugiperda" class="mw-redirect" title="Spodoptera frugiperda">Spodoptera frugiperda</a>, are used as host. A cell line derived from the <a href="Cabbage_looper" title="Cabbage looper">cabbage looper</a> is of particular interest, as it has been developed to grow fast and without the expensive serum normally needed to boost cell growth.<a href="#cite_note-27">[27]</a><a href="#cite_note-28">[28]</a> The <a href="Shuttle_vector" title="Shuttle vector">shuttle vector</a> is called bacmid, and gene expression is under the control of a strong promoter pPolh.<a href="#cite_note-29">[29]</a> Baculovirus has also been used with mammalian cell lines in the <a href="BacMam" title="BacMam">BacMam</a> system.<a href="#cite_note-Kost2002-30">[30]</a>

Baculovirus is normally used for production of <a href="Glycoproteins" class="mw-redirect" title="Glycoproteins">glycoproteins</a>, although the glycosylations may be different from those found in vertebrates. In general, it is safer to use than mammalian virus as it has a limited host range and does not infect vertebrates without modifications.

<div class="mw-heading mw-heading3"><h3 id="Plant">Plant</h3></div>

Many plant expression vectors are based on the <a href="Ti_plasmid" title="Ti plasmid">Ti plasmid</a> of <a href="Agrobacterium_tumefaciens" title="Agrobacterium tumefaciens">Agrobacterium tumefaciens</a>.<a href="#cite_note-31">[31]</a> In these expression vectors, DNA to be inserted into plant is cloned into the <a href="T-DNA_Binary_system" class="mw-redirect" title="T-DNA Binary system">T-DNA</a>, a stretch of DNA flanked by a 25-bp direct repeat sequence at either end, and which can integrate into the plant genome. The T-DNA also contains the selectable marker. The Agrobacterium provides a mechanism for <a href="Transformation_(genetics)" class="mw-redirect" title="Transformation (genetics)">transformation</a>, integration of into the plant genome, and the promoters for its vir genes may also be used for the cloned genes. Concerns over the transfer of bacterial or viral genetic material into the plant however have led to the development of vectors called intragenic vectors whereby functional equivalents of plant genome are used so that there is no transfer of genetic material from an alien species into the plant.<a href="#cite_note-32">[32]</a>

Plant viruses may be used as vectors since the Agrobacterium method does not work for all plants. Examples of plant virus used are the <a href="Tobacco_mosaic_virus" title="Tobacco mosaic virus">tobacco mosaic virus</a> (TMV), <a href="Potato_virus_X" title="Potato virus X">potato virus X</a>, and <a href="Cowpea_mosaic_virus" title="Cowpea mosaic virus">cowpea mosaic virus</a>.<a href="#cite_note-33">[33]</a> The protein may be expressed as a fusion to the coat protein of the virus and is displayed on the surface of assembled viral particles, or as an unfused protein that accumulates within the plant. Expression in plant using plant vectors is often constitutive,<a href="#cite_note-34">[34]</a> and a commonly used constitutive promoter in plant expression vectors is the <a href="Cauliflower_mosaic_virus" title="Cauliflower mosaic virus">cauliflower mosaic virus</a> (CaMV) 35S promoter.<a href="#cite_note-35">[35]</a><a href="#cite_note-36">[36]</a>

<div class="mw-heading mw-heading3"><h3 id="Mammalian">Mammalian</h3></div>

Mammalian expression vectors offer considerable advantages for the expression of mammalian proteins over bacterial expression systems - proper folding, post-translational modifications, and relevant enzymatic activity. It may also be more desirable than other eukaryotic non-mammalian systems whereby the proteins expressed may not contain the correct glycosylations. It is of particular use in producing membrane-associating proteins that require chaperones for proper folding and stability as well as containing numerous post-translational modifications. The downside, however, is the low yield of product in comparison to prokaryotic vectors as well as the costly nature of the techniques involved. Its complicated technology, and potential contamination with animal viruses of mammalian cell expression have also placed a constraint on its use in large-scale industrial production.<a href="#cite_note-mammalian-37">[37]</a>

Cultured mammalian cell lines such as the <a href="Chinese_hamster_ovary_cell" title="Chinese hamster ovary cell">Chinese hamster ovary (CHO)</a>, <a href="COS_cells" title="COS cells">COS</a>, including human cell lines such as <a href="HEK_cell" class="mw-redirect" title="HEK cell">HEK</a> and <a href="HeLa" title="HeLa">HeLa</a> may be used to produce protein. Vectors are <a href="Transfected" class="mw-redirect" title="Transfected">transfected</a> into the cells and the DNA may be integrated into the genome by <a href="Homologous_recombination" title="Homologous recombination">homologous recombination</a> in the case of stable transfection, or the cells may be transiently transfected. Examples of mammalian expression vectors include the <a href="Adenoviral" class="mw-redirect" title="Adenoviral">adenoviral</a> vectors,<a href="#cite_note-38">[38]</a> the pSV and the pCMV series of plasmid vectors, <a href="Vaccinia" title="Vaccinia">vaccinia</a> and <a href="Retroviral" class="mw-redirect" title="Retroviral">retroviral</a> vectors,<a href="#cite_note-39">[39]</a> as well as baculovirus.<a href="#cite_note-Kost2002-30">[30]</a> The promoters for <a href="Cytomegalovirus" title="Cytomegalovirus">cytomegalovirus</a> (CMV) and <a href="SV40" title="SV40">SV40</a> are commonly used in mammalian expression vectors to drive gene expression. Non-viral promoter, such as the elongation factor (EF)-1 promoter, is also known.<a href="#cite_note-40">[40]</a>

<div class="mw-heading mw-heading3"><h3 id="Cell-free_systems">Cell-free systems</h3></div>

E. coli <a href="Cell_lysate" class="mw-redirect" title="Cell lysate">cell lysate</a> containing the cellular components required for transcription and translation are used in this in vitro method of protein production. The advantage of such system is that protein may be produced much faster than those produced in vivo since it does not require time to culture the cells, but it is also more expensive. Vectors used for E. coli expression can be used in this system although specifically designed vectors for this system are also available. Eukaryotic cell extracts may also be used in other cell-free systems, for example, the <a href="Wheat_germ" class="mw-redirect" title="Wheat germ">wheat germ</a> cell-free expression systems.<a href="#cite_note-41">[41]</a> Mammalian cell-free systems have also been produced.<a href="#cite_note-42">[42]</a>

<div class="mw-heading mw-heading2"><h2 id="Applications">Applications</h2></div>

<div class="mw-heading mw-heading3"><h3 id="Laboratory_use">Laboratory use</h3></div>

Expression vector in an expression host is now the usual method used in laboratories to produce proteins for research. Most proteins are produced in E. coli, but for glycosylated proteins and those with disulphide bonds, yeast, baculovirus and mammalian systems may be used.

<div class="mw-heading mw-heading3"><h3 id="Production_of_peptide_and_protein_pharmaceuticals">Production of peptide and protein pharmaceuticals</h3></div>

Most protein <a href="Pharmaceuticals" class="mw-redirect" title="Pharmaceuticals">pharmaceuticals</a> are now produced through recombinant DNA technology using expression vectors. These peptide and protein pharmaceuticals may be hormones, vaccines, antibiotics, antibodies, and enzymes.<a href="#cite_note-pharmaceutical-43">[43]</a> The first human recombinant protein used for disease management, insulin, was introduced in 1982.<a href="#cite_note-pharmaceutical-43">[43]</a> Biotechnology allows these peptide and protein pharmaceuticals, some of which were previously rare or difficult to obtain, to be produced in large quantity. It also reduces the risks of contaminants such as host viruses, toxins and <a href="Prions" class="mw-redirect" title="Prions">prions</a>. Examples from the past include <a href="Prion" title="Prion">prion</a> contamination in <a href="Growth_hormone" title="Growth hormone">growth hormone</a> extracted from <a href="Pituitary_gland" title="Pituitary gland">pituitary glands</a> harvested from human cadavers, which caused <a href="Creutzfeldt%E2%80%93Jakob_disease" title="Creutzfeldt–Jakob disease">Creutzfeldt–Jakob disease</a> in patients receiving treatment for <a href="Dwarfism" title="Dwarfism">dwarfism</a>,<a href="#cite_note-44">[44]</a> and viral contaminants in clotting <a href="Factor_VIII" title="Factor VIII">factor VIII</a> isolated from human blood that resulted in the transmission of viral diseases such as <a href="Hepatitis" title="Hepatitis">hepatitis</a> and <a href="AIDS" class="mw-redirect" title="AIDS">AIDS</a>.<a href="#cite_note-45">[45]</a><a href="#cite_note-46">[46]</a> Such risk is reduced or removed completely when the proteins are produced in non-human host cells.

<div class="mw-heading mw-heading3"><h3 id="Transgenic_plant_and_animals">Transgenic plant and animals</h3></div>

In recent years, expression vectors have been used to introduce specific genes into plants and animals to produce <a href="Transgenic" class="mw-redirect" title="Transgenic">transgenic</a> organisms, for example in <a href="Agriculture" title="Agriculture">agriculture</a> it is used to produce <a href="Transgenic_plants" class="mw-redirect" title="Transgenic plants">transgenic plants</a>. Expression vectors have been used to introduce a <a href="Vitamin_A" title="Vitamin A">vitamin A</a> precursor, <a href="Beta-carotene" class="mw-redirect" title="Beta-carotene">beta-carotene</a>, into rice plants. This product is called <a href="Golden_rice" title="Golden rice">golden rice</a>. This process has also been used to introduce a gene into plants that produces an <a href="Insecticide" title="Insecticide">insecticide</a>, called <a href="Bacillus_thuringiensis" title="Bacillus thuringiensis">Bacillus thuringiensis toxin</a> or <a href="Bacillus_thuringiensis" title="Bacillus thuringiensis">Bt toxin</a> which reduces the need for farmers to apply insecticides since it is produced by the modified organism. In addition expression vectors are used to extend the ripeness of tomatoes by altering the plant so that it produces less of the chemical that causes the tomatoes to rot.<a href="#cite_note-47">[47]</a> There have been <a href="Genetically_modified_food_controversies" title="Genetically modified food controversies">controversies</a> over using expression vectors to modify crops due to the fact that there might be unknown health risks, possibilities of companies patenting certain <a href="Genetically_modified_food" title="Genetically modified food">genetically modified food</a> crops, and ethical concerns. Nevertheless, this technique is still being used and heavily researched.

<a href="Transgenic_animals" class="mw-redirect" title="Transgenic animals">Transgenic animals</a> have also been produced to study animal biochemical processes and human diseases, or used to produce pharmaceuticals and other proteins. They may also be engineered to have advantageous or useful traits. <a href="Green_fluorescent_protein" title="Green fluorescent protein">Green fluorescent protein</a> is sometimes used as tags which results in animal that can fluoresce, and this have been exploited commercially to produce the fluorescent <a href="GloFish" title="GloFish">GloFish</a>.

<div class="mw-heading mw-heading3"><h3 id="Gene_therapy">Gene therapy</h3></div>

<div role="note" class="hatnote navigation-not-searchable">Main article: <a href="Vectors_in_gene_therapy" title="Vectors in gene therapy">Vectors in gene therapy</a></div>

<a href="Gene_therapy" title="Gene therapy">Gene therapy</a> is a promising treatment for a number of diseases where a "normal" gene carried by the vector is inserted into the genome, to replace an "abnormal" gene or supplement the expression of particular gene. Viral vectors are generally used but other nonviral methods of delivery are being developed. The treatment is still a risky option due to the viral vector used which can cause ill-effects, for example giving rise to <a href="Insertional_mutation" class="mw-redirect" title="Insertional mutation">insertional mutation</a> that can result in cancer.<a href="#cite_note-48">[48]</a><a href="#cite_note-49">[49]</a> However, there have been promising results.<a href="#cite_note-50">[50]</a><a href="#cite_note-51">[51]</a>

<ul><li><a href="Cloning_vector" title="Cloning vector">Cloning vector</a></li>

<li><a href="Host_cell_protein" title="Host cell protein">Host cell protein</a></li></ul>

<div class="mw-heading mw-heading2"><h2 id="References">References</h2></div>

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<div class="mw-heading mw-heading2"><h2 id="External_links">External links</h2></div>

<ul><li><a rel="nofollow" class="external text" href="http://www.gelifesciences.com/handbooks">GST Gene Fusion System Handbook</a> <a rel="nofollow" class="external text" href="https://web.archive.org/web/20081205061748/http://www.gelifesciences.com/handbooks">Archived</a> 2008-12-05 at the <a href="Wayback_Machine" title="Wayback Machine">Wayback Machine</a></li></ul></div><div><div class="zim-footer">

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